Abstract of Study (Max 300 Words): _Objective/Objectives: The objective of the present work was to overexpress HGF gene in non HGF producing humane myeloma cell lines (INA-6).
Materials and Methods:. In this research, retroviral- expression constructs were designed and constructed. The sequence of the cloned fragments in the vector constructs was determined and the effect of construct was examined using qRT-PCR and Western blot analysis.
Results: We established expression constructs based on HGF mRNA sequence. The HGF non producing human myeloma cell line INA-6 transduced with lentiviral HGF-overexpression transduction particles, were analyzed for their effect on the HGF gene expression. HGF overexpression was analyzed both at mRNA level and protein levels. Two myeloma cell line INA-6 HGF-1 and INA-6HGF-2 showed significant over expression of HGF mRNA and protein.
Conclusion/Conclusions: In conclusion, the HGF overexpression cell lines can be used as tissue culture models to investigate the role of HGF protein in pathology of multiple myeloma.
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